dart

Diversity Arrays Technology (DArT) is a high-throughput genetic marker technique that can detect allelic variations to provides comprehensive genome coverage without any DNA sequence information for genotyping and other genetic analysis. The general steps involve reducing the complexity of the genomic DNA with specific restriction enzymes, choosing diverse fragments to serve as representations for the parent genomes, amplify via polymerase chain reaction (PCR), insert fragments into a vector to be placed as probes within a microarray, then fluorescent targets from a reference sequence will be allowed to hybridize with probes and put through an imaging system. The objective is to identify and quantify various forms of DNA polymorphism within genomic DNA of sampled species.First reported in 2001 by Damian Jaccoud, Andrzej Kilian, David Feinstein, and Kaiman Peng, DArT prioritized significant advantages over other traditional primer-based methods like the ability to analyze large amounts of various samples from a low amount of initial DNA. It also afforded low costs and faster results compared to related solid state DNA arrays that detected Single Nucleotide Polymorphisms (SNPs). Since its inception, the technology has been a major instrument in the analysis of polyploid plants as well as in the construction of physical and genetic map to understand related on species based on similarities and allelic variances among their genomes.

View More On Wikipedia.org
Recent contents

  1. Mabasi ya Mwendokasi kufika hadi Chamazi

    Wakati mkandarasi mradi awamu ya tatu ya mabasi yaendayo haraka ’mwendokasi’ barabara ya Gongo la Mboto akiwa tayari ameanza kazi, miundombinu ya mradi huo inatarajiwa kufika hadi Chamazi. Hayo yamesemwa leo Jumanne Novemba 15,2022 jijini humo na Mtendaji Mkuu wa Wakala wa Mabasi Yaendayo...

  2. DAR: DART kuondoa tiketi vituo vya Mwendokasi, malipo ya kadi kurejea

    Kampuni ya Emirates National Group kutoka Falme za Kiarabu iliyopata zabuni ya kuendesha mradi wa Mabasi hayo itaanza utaratibu huo Novemba 2022. Pia, Mkurugenzi wa Maendeleo ya Usafirishaji wa DART, Fanuel Karugendo amesema wameipa miezi 6 kampuni hiyo kuingiza mabasi mapya na kwa kuanza...

  3. Prof. Tumaini Joseph Nagu ateuliwa Kuwa Mganga Mkuu wa Serikali, Dkt. Florens Martin Turuka bosi mpya DART

    Rais wa Jamhuri ya Muungano wa Tanzania, Samia Suluhu Hassan, amemteua Dkt. Florens Martin Turuka, Katibu Mkuu Mstaafu kuwa Mwenyekiti wa Bodi ya Wakala wa Mabasi Yaendayo Haraka (DART). Amemteua Bi. Beng’i Mazana Issa kuwa Mwenyekiti wa Bodi ya Mikopo ya Serikali za Mitaa. Bi. Beng'i ni...

  4. Barabara za Mwendokasi zinadharaulika na wenye mamlaka wenyewe

    Matukio ya magari yasiyohusika kupita kwenye barabara ya mwendo wa haraka, yameibua hisia za kudharau utendaji wa mradi wa mabasi yaendayo haraka (BRT) na Sheria ya Usalama Barabarani. Uvunjifu wa sheria na taratibu zilizowekwa umeonekana kufanywa zaidi na magari ya vyombo vya usalama, magari...
    • BARD AI
    • Thread
    • barabara dart mamlaka mwendokasi serikali wenye wenyewe
    • Replies: 10
    • Forum: Jukwaa la Siasa

  5. DART: Magari pekee yanayoruhusiwa kutumia njia za Mwendokasi

Menu
Log in
Register
Cookies are required to use this site. You must accept them to continue using the site. Learn more…