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Steven Gerrard accused Mario Balotelli of showing "disrespect" by taking Liverpool's winning penalty against Besiktas ahead of Jordan Henderson.
Balotelli scored from the spot in Thursday's Europa League last-32 tie at Anfield to secure a 1-0 first-leg win.
That came after a disagreement with captain Henderson and Daniel Sturridge over who should take it.
Gerrard said: "Henderson is the captain and Balotelli showed Jordan a bit of disrespect there."
The midfielder, who is currently injured, was working as a pundit on ITV's coverage of the game.
Gerrard said that Balotelli was being "a bit mischievous" after the Italian striker took the ball off Henderson, who was set to to take the spot kick.
Henderson stood in as Liverpool's captain against Besiktas, and Gerrard believes he should have taken the penalty.
"Rules are rules," he said. "Credit to Mario, he's scored, but it's not nice to see when footballers are arguing. I think Jordan has handled the situation very well."
Liverpool boss Brendan Rodgers would not clarify who should have taken the penalty after the game.
How the incident unfolded
With less than 10 minutes to go, the impressive Jordon Ibe was fouled inside the area and referee Szymon Marciniak immediately awarded the penalty.
Henderson picked up the ball and walked towards the spot...
What now boss?
...Balotelli took the ball from Henderson and appeared to get in a disagreement with both the Liverpool midfielder and striker Daniel Sturridge. Henderson looked over to the bench...
I've only missed two
...Balotelli had scored 26 of his 28 penalties before Thursday's game and appeared determined to take the spot kick, placing the ball on the penalty spot...
..and scored.
Best of friends
Balotelli celebrated with his team-mates including Henderson, who initially acknowledged the goal by tapping the striker on shoulder, before Balotelli pulled him back for a hug.
"It was a big goal," Henderson told ITV.
"I wanted the penalty, Mario felt confident and he has taken big penalties before. Everyone wants to take them. I felt confident in Mario, he has taken big penalties before and he got the nod."
Balotelli posted on Instagram after the game: (external) "Thank you hendo for let me take the penalty.. Stop drama now. We won that's what it count. We are a team and especially we are Liverpool. Come on guys."
Asked to clarify who should have taken the penalty after the game, Rodgers said: "I am clarifying we won the game 1-0.
"I have a number of outstanding penalty takers with Jordan [Henderson] and Mario [Balotelli] and Rickie Lambert.
"It was the perfect result and sets up for the second leg next week."
Ndo Tatizo la waingereza, SG naye kajigeuza kama mungu pale liver, MB ni mpiga penati mzuri na kafunga penati ya muhimu, sasa sijui kelele zinatoka wapi.
Jamie Rednap naye eti anasema kutokana na dharau ya MB kwa kapteni na wachezaji wenzake, haoni akichezea Liver msimu ujao! Uingereza na kuifanya Liver ndo kama timu ya waingereza kunaiponza sana Liver. Mwaka jana uk media walithubutu kusema SG ANASTAHILI kushinda lile kombe la EPL la msimu uliopita na sio Vincent Company au Yaya Toure au Hazard, ila yeye SG ndo alijikwaa vs Chelsea na sasa EPL title ataisikia kwenye bomba tuu. Huwa najaribu kuipenda Liver, ila waingereza wananifanya NISIIPENDE sababu wako biased nayo sana, hasa media.
SG ni mchezaji mzuri wa Uingereza, ni legenda pale liver, ila asianze kuropoka.
Kwangu mimi, naona Martin Skartel ni nahodha mzuri zaidi ya Hendo. Zama zimepita, mpaka watoe uingereza ktk Liver, wataendelea kuwa an average team at it's best, mambo ya kusema ni timu kubwa based on 1980s history ni wendawazimu.
arsenal is making it very difficult, walitakiwa wachapwe leoIli tuingie top4 tunahitaji kushinda mechi zinazoitwa kua ni ngumu kama hii ya ksho yaani inatubidi tuchukue point kwa walio juu yetu. No way out
arsenal is making it very difficult, walitakiwa wachapwe leo
Mkuu Twende ivyo ivyo mi naamini nafasi ya tatu na ya nne so far hazina mtu ukiwatoa City na Chelsea wenye ya kwanza na ya pili
ni kweli mkuu, hako katimu huwa kanaisha gesi mwezi huu, naona this time gesi haiishi