Huyu anafikiria kinachoweza kufanya na hiyo technology ambayo iligunduliwa 1994. Mbona wangelifanya toka miaka hiyo? Conspiracy theories
mNeonGreen: a yellow-green fluorescent protein
mNeonGreen is a yellow-green fluorescent protein that was derived from the tetrameric yellow fluorescent protein (LanYFP) from the marine invertebrate
Branchiostoma lanceolatum in 2012. Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University, and the Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP (EGFP)-type termini.
mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation maxima is at 506 nm and its emission maxima is at 517 nm. Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged either with standard green fluorescent protein filters or yellow fluorescent protein filters, with no or minimal reduction in emission readout, respectively.